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Microscale thermophoresis : ウィキペディア英語版
Microscale thermophoresis
MicroScale Thermophoresis (MST) is a technology for the interaction analysis of biomolecules. MicroScale Thermophoresis is the directed movement of particles in a microscopic temperature gradient. Any change of the hydration shell of biomolecules due to changes in their structure/conformation results in a relative change of the movement along the temperature gradient and is used to determine binding affinities. MST allows measurement of interactions directly in solution without the need of immobilization to a surface (immobilization-free technology). MicroScale Thermophoresis was developed by the NanoTemper Technologies GmbH, a German high tech company with headquarters in Munich.
== Applications ==

Affinity
* between any kind of biomolecules including proteins, DNA, RNA, peptides, small molecules, fragments and ions
* for interactions with high molecular weight complexes, large molecule assemblies, even with liposomes, vesicles, nanodiscs, nanoparticles and viruses
* in any buffer, including serum and cell lysate
* in competition experiments (for example with subtrate and inhibitors)
Stoichiometry
Thermodynamic parameters
Additional information
* Sample property (homogeneity, aggregation, stability)
* Multiple binding sites, cooperativity

抄文引用元・出典: フリー百科事典『 ウィキペディア(Wikipedia)
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